{"id":20942,"date":"2026-04-14T11:38:34","date_gmt":"2026-04-14T10:38:34","guid":{"rendered":"https:\/\/cabd.es\/servicio-de-citrometria\/"},"modified":"2026-06-01T13:46:24","modified_gmt":"2026-06-01T12:46:24","slug":"servicio-de-citrometria","status":"publish","type":"page","link":"https:\/\/cabd.es\/en\/services\/servicio-cientifico-tecnico\/servicio-de-citrometria\/","title":{"rendered":"Cytometry"},"content":{"rendered":"

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Scientific-Technical Facilities<\/p>\n

Cytometry<\/h1>\n

[\/et_pb_fullwidth_header][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ _module_preset=”default” custom_margin=”||||false|false” custom_padding=”||0px||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.6″ _module_preset=”dc6ab383-11a3-454b-8ba1-6554be6042b4″ vertical_offset_tablet=”0″ horizontal_offset_tablet=”0″ z_index_tablet=”0″ text_text_shadow_horizontal_length_tablet=”0px” text_text_shadow_vertical_length_tablet=”0px” text_text_shadow_blur_strength_tablet=”1px” link_text_shadow_horizontal_length_tablet=”0px” link_text_shadow_vertical_length_tablet=”0px” link_text_shadow_blur_strength_tablet=”1px” ul_text_shadow_horizontal_length_tablet=”0px” ul_text_shadow_vertical_length_tablet=”0px” ul_text_shadow_blur_strength_tablet=”1px” ol_text_shadow_horizontal_length_tablet=”0px” ol_text_shadow_vertical_length_tablet=”0px” ol_text_shadow_blur_strength_tablet=”1px” quote_text_shadow_horizontal_length_tablet=”0px” quote_text_shadow_vertical_length_tablet=”0px” quote_text_shadow_blur_strength_tablet=”1px” header_text_shadow_horizontal_length_tablet=”0px” header_text_shadow_vertical_length_tablet=”0px” header_text_shadow_blur_strength_tablet=”1px” header_2_text_shadow_horizontal_length_tablet=”0px” header_2_text_shadow_vertical_length_tablet=”0px” header_2_text_shadow_blur_strength_tablet=”1px” header_3_text_shadow_horizontal_length_tablet=”0px” header_3_text_shadow_vertical_length_tablet=”0px” header_3_text_shadow_blur_strength_tablet=”1px” header_4_text_shadow_horizontal_length_tablet=”0px” header_4_text_shadow_vertical_length_tablet=”0px” header_4_text_shadow_blur_strength_tablet=”1px” header_5_text_shadow_horizontal_length_tablet=”0px” header_5_text_shadow_vertical_length_tablet=”0px” header_5_text_shadow_blur_strength_tablet=”1px” header_6_text_shadow_horizontal_length_tablet=”0px” header_6_text_shadow_vertical_length_tablet=”0px” header_6_text_shadow_blur_strength_tablet=”1px” box_shadow_horizontal_tablet=”0px” box_shadow_vertical_tablet=”0px” box_shadow_blur_tablet=”40px” box_shadow_spread_tablet=”0px” global_colors_info=”{}”]<\/p>\n

1. Service definition<\/h2>\n

 <\/p>\n

\n
\n

Flow cytometry is a powerful technique for the analysis of multiple parameters of individual cells within heterogeneous populations. The flow cytometers performs this analysis by passing thousands of cells per second through a laser beam and capturing the light that emerges from each cell as it passes through. The data gathered can be analyzed statistically by flow cytometry softwares to report cellular characteristics such as:<\/p>\n

    \n
  • Cell size (FSC)<\/li>\n
  • Cell complexity (SSC)<\/li>\n
  • Fluorescence<\/li>\n<\/ul>\n

    Flow cytometers have the ability to measure multiple celular parameters and markers of a large number of cells at a rapid rate. So the applications of flow cytometry are numerous, and this has led to the widespread use of these instruments in biological research.
    The main applications that we have developed in the center are:<\/p>\n

      \n
    • Cell viability assays<\/li>\n
    • Membrane potencial<\/li>\n
    • Inmunophenotyping<\/li>\n
    • Cell cycle analysis<\/li>\n
    • Apoptosis and necrosis<\/li>\n
    • Cell sorting\n
        \n
      • Stem cells (mouse)<\/li>\n
      • Cell lines<\/li>\n
      • Drosophila discs cells (eyes and wings)<\/li>\n
      • Hematopoietic cells (mouse blood, kidney, liver, bone marrow, spleen, thymus)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n

         <\/p>\n<\/div>\n<\/div>\n

        2. Offered services<\/h2>\n

         <\/p>\n

          \n
        • Assisting researchers in designing experiments<\/li>\n
        • Analytical cytometers’ user training<\/li>\n
        • Assisting researchers with analysis and data interpretation<\/li>\n
        • Sorting cell populations<\/li>\n<\/ul>\n

          <\/h3>\n

          3. Personnel and contact<\/h2>\n

           <\/p>\n

            \n
          • M\u00aa Jos\u00e9 S\u00e1nchez Sanz-\u00a0 Scientific Coordinator<\/li>\n
          • Cor\u00edn D\u00edaz Ramos- Head Technician. CSIC.\u00a0 954977432<\/li>\n
          • Macarena L\u00f3pez Mayorga-Technician<\/li>\n<\/ul>\n

            4. Equipament<\/h2>\n

             <\/p>\n

            \u00a0Sony MA900 Cell Sorter<\/span><\/h3>\n
              \n
            • Four lasers on two axes: 488\/561nm and 405\/638 nm<\/li>\n
            • 14 parameters: FSC, BSC and 12 fluorescence colours<\/li>\n
            • Sample acquisition tube types: 0,5ml, 1.5ml, 5ml and 15ml<\/li>\n
            • Acquisition rates of up to 70 000 events per second<\/li>\n
            • Temperature control of sample and collection tubes: 5oC and 37oC<\/li>\n
            • Cell sorting in 2-way and 4-way tubes, epp or 6,12,24,48,96 & 384- well plates<\/li>\n
            • Index sorting software<\/li>\n
            • Nozzles: 70 and 100 micron<\/li>\n
            • Software: SONY-MA (online\/offline)<\/li>\n<\/ul>\n

              \u00a0The available optic configuration is detailed below:<\/h4>\n
              \n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
              L\u00e1ser<\/strong><\/td>\nFiltros \u00f2pticos<\/strong><\/td>\nLPF<\/strong><\/td>\nFiltros BP<\/strong><\/td>\nFluorocromos<\/strong><\/td>\n<\/tr>\n
              405nm<\/strong><\/td>\nFL6<\/td>\n<\/td>\n450\/50<\/td>\nDapi, Bviolet 421<\/td>\n<\/tr>\n
              FL7\/LP10<\/td>\n487.5LP<\/td>\n525\/50<\/td>\nBViolet510,Pacific Orange<\/td>\n<\/tr>\n
              FL8\/LP4<\/td>\n561LP<\/td>\n585\/30<\/td>\nBViolet570<\/td>\n<\/tr>\n
              FL9\/LP3<\/td>\n600LP<\/td>\n617\/30<\/td>\nBViolet 605<\/td>\n<\/tr>\n
              FL10\/LP7<\/td>\n639LP<\/td>\n665\/30<\/td>\nBViolet 650<\/td>\n<\/tr>\n
              FL11\/LP6<\/td>\n685LP<\/td>\n720\/60<\/td>\nBViolet 711<\/td>\n<\/tr>\n
              FL12\/LP5<\/td>\n752LP<\/td>\n785\/60<\/td>\nBViolet 785<\/td>\n<\/tr>\n
              488nm<\/strong><\/td>\nFL1<\/td>\n<\/td>\n525\/50<\/td>\nFitc, AlexaFluor 488,EGFP,EYFP<\/td>\n<\/tr>\n
              FL2\/LP4<\/td>\n561LP<\/td>\n585\/30<\/td>\nPE<\/td>\n<\/tr>\n
              FL3\/LP3<\/td>\n600LP<\/td>\n617\/30<\/td>\nPE-Texas Red, PI,<\/td>\n<\/tr>\n
              FL4\/LP7<\/td>\n639LP<\/td>\n695\/50<\/td>\n7AAD, PE-cy5, PerCP<\/td>\n<\/tr>\n
              FL5\/LP1<\/td>\n752LP<\/td>\n785\/60<\/td>\nPE-CY7<\/td>\n<\/tr>\n
              BSC<\/td>\n<\/td>\n488\/17B<\/td>\n<\/td>\n<\/tr>\n
              561nm<\/strong><\/td>\nFL2\/LP4<\/td>\n561LP<\/td>\n585\/30<\/td>\nmOrange, DsREd-Monomer, tdTomato<\/td>\n<\/tr>\n
              FL3\/LP3<\/td>\n600LP<\/td>\n617\/30<\/td>\nmCherry, Alexa Fluor 594<\/td>\n<\/tr>\n
              FL4\/LP7<\/td>\n639LP<\/td>\n695\/50<\/td>\nmPlum<\/td>\n<\/tr>\n
              638nm<\/strong><\/td>\nFL10\/LP7<\/td>\n639LP<\/td>\n665\/30<\/td>\nAPC, Cy5, Alexa Fluor 647<\/td>\n<\/tr>\n
              FL11\/LP6<\/td>\n685LP<\/td>\n720\/60<\/td>\nAPC-Cy5.5, Alexa Fluor 700<\/td>\n<\/tr>\n
              FL12\/LP5<\/td>\n752LP<\/td>\n785\/60<\/td>\nAPC-Cy7, APC-Alexa Fluor 750<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

               <\/p>\n

              BD FACSAria Cell Sorter<\/span><\/h3>\n

              <\/span><\/strong><\/p>\n

                \n
              • Two lasers: 488 nm y 633 nm<\/li>\n
              • 9 parameters: FSC, SSC and 7 fluorescence colors<\/li>\n
              • Sample acquisition with temperature control<\/li>\n
              • Acquisition rates of up to 70 000 events per seconds<\/li>\n
              • Sorts of up to four populations simultaneously<\/li>\n
              • Cell sorting in tube or culture plate<\/li>\n
              • Nozzles: 70 and 100 micron<\/li>\n
              • Software: FACSDiva<\/li>\n<\/ul>\n

                \u00a0The available optic configuration is detailed below:<\/h4>\n
                \n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
                L\u00e1ser<\/strong><\/td>\nPMT<\/strong><\/td>\nLPF<\/strong><\/td>\nFiltros BP<\/strong><\/td>\nFluorocromos<\/strong><\/td>\n<\/tr>\n
                \u00a0488 nm<\/strong><\/td>\nA<\/strong><\/td>\n735<\/td>\n780\/60<\/td>\nPE-Cy7<\/td>\n<\/tr>\n
                B<\/strong><\/td>\n655<\/td>\n695\/40<\/td>\nPerCP-Cy5.5 \u00f3 PI<\/td>\n<\/tr>\n
                675\/20<\/td>\nPerCP \u00f3 PE-Cy5<\/td>\n<\/tr>\n
                C<\/strong><\/td>\n595<\/td>\n610\/20<\/td>\nPE-Texas Red<\/td>\n<\/tr>\n
                D<\/strong><\/td>\n556<\/td>\n576\/26<\/td>\nPE \u00f3 PI<\/td>\n<\/tr>\n
                585\/42<\/td>\nPE\/PI<\/td>\n<\/tr>\n
                E<\/strong><\/td>\n502<\/td>\n530\/30<\/td>\nFITC<\/td>\n<\/tr>\n
                F<\/strong><\/td>\n<\/td>\n488\/10<\/td>\nSSC<\/td>\n<\/tr>\n
                \u00a0\u00a0633 nm<\/strong><\/td>\nA<\/strong><\/td>\n735<\/td>\n780\/60<\/td>\nAPC-Cy7<\/td>\n<\/tr>\n
                B<\/strong><\/td>\n685<\/td>\n712\/21<\/td>\nAPC-Cy5.5<\/td>\n<\/tr>\n
                C<\/strong><\/td>\n660\/20<\/td>\n660\/20<\/td>\nAPC<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

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                5. Rates<\/h2>\n
                \n

                 <\/p>\n\n\n\n\n\n\n\n\n
                \n
                Offered services<\/strong><\/div>\n

                 <\/p>\n<\/td>\n

                		RATES<\/strong><\/td>\n<\/tr>\n
                UPO<\/strong><\/td>\nUniversity and OPIs<\/strong><\/td>\nEnterprises\/Others<\/strong><\/td>\n<\/tr>\n
                FacsAria Analysis<\/td>\n7,60 \u20ac\/h.<\/td>\n23,00 \u20ac\/h.<\/td>\n30,50 \u20ac\/h.<\/td>\n<\/tr>\n
                FacsAria Cell sorter<\/td>\n10,15 \u20ac\/h.<\/td>\n30,50 \u20ac\/h.<\/td>\n40,60 \u20ac\/h.<\/td>\n<\/tr>\n
                Sony MA900 Analysis<\/td>\n7,60 \u20ac\/h.<\/td>\n23,00 \u20ac\/h.<\/td>\n30,50 \u20ac\/h.<\/td>\n<\/tr>\n
                Sony MA900 Cell sorter<\/td>\n10,15 \u20ac\/h.<\/td>\n30,50 \u20ac\/h.<\/td>\n40,60 \u20ac\/h.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

                 <\/p>\n<\/div>\n

                 <\/p>\n

                6. Publications and web resources<\/h2>\n

                <\/h3>\n

                Web resources<\/h3>\n

                1. Invitrogen: Fluorescence Tutorials
                www.invitrogen.com\/site\/us\/en\/home\/support\/Tutorials.html<\/a><\/p>\n

                2. BD Biosciences: Spectrum Viewer
                https:\/\/www.bdbiosciences.com\/en-us\/resources\/bd-spectrum-viewer<\/a><\/p>\n

                3. Purdue University Cytometry Laboratories
                www.cyto.purdue.edu\/<\/a><\/p>\n

                4. Iberian Cytometry Society
                www.citometria.org\/<\/a><\/p>\n

                5. Compensation tutorial
                http:\/\/drmr.com\/<\/a><\/p>\n

                6. Web tool for flow cytometry data analysis. (For questions, you may consult:\u00a0https:\/\/umgcccfcsr.github.io\/CytometryInR\/course\/00_Floreada\/<\/a>\u00a0or ask Macarena L\u00f3pez.)<\/p>\n

                https:\/\/floreada.io\/<\/a><\/strong><\/p>\n

                Flow basics 2.1: https:\/\/www.youtube.com\/watch?v=gC4brjHbLkk Conceptos b\u00e1sicos sobre preparaci\u00f3n de la muestra<\/p>\n

                Flow basics 2.2:https:\/\/www.youtube.com\/watch?v=SXyeDKZecZA. Preparaci\u00f3n de la muestra en m\u00e1s profundidad: digesti\u00f3n de la muestra: m\u00e9todo de digesti\u00f3n, elecci\u00f3n de enzima de digesti\u00f3n, titulaci\u00f3n de anticuerpos, cantidad de c\u00e9lulas y c\u00e1lculo del \u00edndice de tinci\u00f3n.<\/p>\n

                 <\/p>\n

                El resto de v\u00eddeos est\u00e1n m\u00e1s relacionados con la realizaci\u00f3n de la citometr\u00eda:<\/p>\n

                Flow basics 2.3:\u00a0https:\/\/www.youtube.com\/watch?v=WHtqy3X_ch8<\/a>
                Flow basics 2.4:\u00a0                https:\/\/www.youtube.com\/watch?v=b0MVzjeTzbk<\/a>
                Flow basics 2.5:\u00a0                https:\/\/www.youtube.com\/watch?v=wTUviuik6rw<\/a><\/p>\n

                 <\/p>\n

                 <\/p>\n

                 <\/p>\n

                Publications<\/h3>\n
                  \n
                1. Flow cytometry and cell sorting (1992) A. Radbruch
                  Springer-Verlag, Berlin.
                  (579 FLO)<\/li>\n
                2. In living color: protocols in flow cytometry Rochelle A. Diamond, Susan DeMaggio
                  (579 IN)<\/li>\n
                3. Flow Cytometry. A Practical Approach (2000) Ormerod, MG.
                  3rd edition. Oxford University Press, Oxford. Robinson, J.P.,
                  (576 FLO)<\/li>\n
                4. Practical Flow Cytometry (2003) Howard Shapiro.
                  Fourth Edition\u00a0 Wiley-Liss, New York
                  (576 SHA)<\/li>\n
                5. Cytometry: new developments (2004)San Diego: Elsevier
                  (576 CYT)<\/li>\n
                6. \u00a0Flow cytometry protocols (2004)
                  Totowa, New Jersey, Humana Press, cop.
                  (576 HAW flo)<\/li>\n
                7. Flow cytometry: principles and applications (2007)
                  Totowa, New Jersey, Humana Press, cop.
                  (576 FLO)<\/li>\n<\/ol>\n

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