{"id":21418,"date":"2026-04-29T09:50:05","date_gmt":"2026-04-29T08:50:05","guid":{"rendered":"https:\/\/cabd.es\/services\/servicio-cientifico-tecnico\/cell-culture-facility\/"},"modified":"2026-09-28T09:07:35","modified_gmt":"2026-09-28T08:07:35","slug":"cell-culture-facility","status":"publish","type":"page","link":"https:\/\/cabd.es\/en\/services\/servicio-cientifico-tecnico\/cell-culture-facility\/","title":{"rendered":"Cell Culture Facility"},"content":{"rendered":"

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Scientific-Technical Facilities<\/p>\n

Cell Culture Facility<\/h1>\n

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\u00a01. Service definition<\/h2>\n
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The CABD Cell Culture Facility<\/strong> provides technical and scientific support to research groups in the development of experimental procedures involving primary human and mouse cell cultures, established cell lines, and three-dimensional (3D) cellular models<\/strong>.<\/p>\n

The service provides a range of specialized facilities for conventional cell culture<\/strong>, as well as for the development, automation, and analysis of stem cell-derived 3D models<\/strong>, including organoids, embryoids, and other complex in vitro models.<\/p>\n

The Service is composed of the following infrastructure:<\/p>\n

a. P2 Cell Culture Room<\/strong><\/p>\n

The P2 Cell Culture Room<\/strong> is intended for work with primary human and mouse cell cultures and established cell lines<\/strong>. It also supports transfection procedures and genetic modification using viral vectors, in accordance with the relevant authorizations and established procedures.<\/p>\n

The room is equipped with the necessary facilities for the generation, maintenance, and manipulation of cell cultures.<\/p>\n

b. White Room for 3D Culture \u2014 Room 129<\/strong><\/p>\n

The White Room (Room 129)<\/strong> is specifically dedicated to the development of three-dimensional (3D) models derived from human and mouse stem cells<\/strong>, including iPSC and hESC cultures<\/strong> for the generation of organoids, embryoids, and other complex 3D models.<\/p>\n

The room provides controlled environmental conditions and is equipped for the culture and maintenance of stem cell-derived 3D models.<\/p>\n

The White Room is not intended for conventional 2D cell culture, cellular reprogramming, transgenesis, or stem cell derivation. The use of primary cells, explants derived from animal models, or organoids generated from primary cultures is not permitted.<\/strong><\/p>\n

c. Automated liquid handling and screening platform –Room 136<\/strong><\/p>\n

The\u00a0 Automated liquid handling and screening platform (Room 136)<\/strong> supports the automation of cell culture, sample handling, image acquisition, and analysis workflows, with a particular focus on 3D cell culture and organoid applications<\/strong>. The platform is also available for 2D and other cell-based applications<\/strong> where automated culture, imaging, analysis, or screening workflows are required. The platform is available for projects involving human or mouse iPSCs and embryonic stem (ES) cells<\/strong>.<\/p>\n

 <\/p>\n

The platform integrates a Cytomat CO\u2082 incubator<\/strong>, a Biomek i5 automated liquid handling system (Beckman Coulter)<\/strong>, and an ImageXpress Nano automated imaging system (Molecular Devices)<\/strong>.<\/p>\n

This infrastructure enables automated and scalable workflows using 96- and 384-well plates, among others<\/strong>, including organoid growth and viability studies, reporter-line assays, and drug screening.<\/p>\n

d. Automated Microscopy Platform \u2014 CellDiscoverer 7<\/strong><\/p>\n

The ZEISS CellDiscoverer 7<\/strong> is a fully automated inverted microscopy system for the acquisition and analysis of live-cell images. It provides control of temperature, CO\u2082, and O\u2082<\/strong>, as well as automated image acquisition over extended periods of time.<\/p>\n

The system enables the monitoring and quantification of a range of cellular processes, including phagocytosis, apoptosis, chemotaxis, migration, invasion, and cytotoxicity<\/strong>, as well as stem cell monitoring and the assessment of transfection and cloning efficiency.<\/p>\n

 <\/p>\n<\/div>\n

\u00a0<\/h3>\n

 <\/p>\n

2. Offered Services<\/h2>\n

 <\/p>\n