Dorothy Hodgkin Proteomics and Biochemistry

1. Service definition

The Dorothy Hodgkin Proteomics and Biochemistry Platform carries out a variety of work and studies on proteins from a multidisciplinary perspective. The services offered range from proteome analysis to on-demand protein purification, including the characterization of intermolecular interactions or the detection and quantification of proteins in biological samples.

2. services offered

3. Personnel and contact

Facility Director

Dra Laura Tomás Gallardo – 954978208

Associated technicians:

Cynthia Alias Villegas

4. Equipamiento

• Sistema de imagen ChemiDoc MP
• Espectrofotómetro UV-Vis Shimadzu UV-1280
• Escáner Image scanner III (Cytiva)
• Escáner Láser Amersham Typhoon RGB (Cytiva)
• Sistemas de electroforesis
• IPGPhor (Cytiva)
• Sonicador Branson SFX 550
• Biacore X100 (Cytiva)
• Nanocalorímetro PEAQ-ITC (MicroCal – Malvern)
• Cabina de flujo laminar Cruma HZ-2
• Microcentrífuga 5415R (Eppendorf)
• Incubado Innova 42 (New Brunswick- Eppendorf)
• Sistema de transferencia Trans-Blot Turbo (BioRad)
• ÄKTA™ Pure 25 FPLC (Cytiva)
• Liofilizador Telstar LyoQuest -85

5. Tarifas

¹ SOLICITAR PRESUPUESTO SEGÚN CARACTERÍSTICAS DEL EXPERIMENTO
² Incluye, lisis, inhibidores de proteasas, limpieza y cuantificación.
³ Incluye electroforesis, transferencia, detección y análisis de imagen. El usuario aportará el anticuerpo.   
⁴ Incluye tinción del gel.

6. Publications and web resources

Publications:

• Fine-Tuning Modulation of Oxidation-Mediated Posttranslational Control of Bradyrhizobium diazoefficiens FixK(2) Transcription Factor. Int. J. Mol. Sci. 2022, 23, 5117. https://doi.org/10.3390/ijms23095117
• Optimized CRISPR-RfxCas13d system for RNA targeting in zebrafish embryos. STAR Protocols, 2022, 3, 101058. https://doi.org/10.1016/j.xpro.2021.101058
• Dissection of FixK(2) protein-DNA interaction unveils new insights into Bradyrhizobium diazoefficiens lifestyles control. Environmental Microbiology, 2021, 23, 6194. https://doi.org/10.1111/1462-2920.15661
• CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos. Developmental Cell, 2020, 54, 805-817. https://doi.org/10.1016/j.devcel.2020.07.013
• The CbrB Regulon: Promoter dissection reveals novel insights into the CbrAB expression network in Pseudomonas putida. PLOS ONE, 2018, 13, e0209191. https://doi.org/10.1371/journal.pone.0209191
• Searching for a Cell-Based Therapeutic Tool for Haemophilia A within the Embryonic/Foetal Liver and the Aorta-Gonads-Mesonephros Region. Thromb. Haemost., 2018, 118, 1370. https://doi.org/10.1055/s-0038-1661351

Web resources:

• Transcription and Translation Tool: Converts sequences from DNA to RNA and from this to protein.

http://biomodel.uah.es/en/lab/cybertory/analysis/trans.htm

• ProtParam tool: allows the computation of various physical and chemical parameters for a given protein.

https://web.expasy.org/protparam/

• SnapGene Viewer: View, annotate and share plasmid sequence files.

https://www.snapgene.com/snapgene-viewer

• Expasy: Swiss Bioinformatics Resource Portal

https://www.expasy.org/

• Uniprot: Base de datos de secuencias de proteínas e información funcional.

https://www.uniprot.org/

• Fold-It: Programa de ciencia ciudadana que permite contribuir a investigaciones científicas mediante una dinámica lúdica de plegamiento de proteínas.

https://fold.it/

• AlphaFold: Base de datos de estructuras de proteínas resueltas por IA.

https://alphafold.ebi.ac.uk/